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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Roles of Three HSF Domain-Containing Proteins in Mediating Heat-Shock Protein Genes and Sustaining Asexual Cycle, Stress Tolerance, and Virulence in Beauveria bassiana
doi: 10.3389/fmicb.2018.01677
Figure Lengend Snippet: Contributions of Hsf1 , Sfl1 , and Skn7 to cell wall integrity of B. bassiana . (A) EC 50 for Congo red to suppress 50% colony growth of each strain after 6-day incubation at 25°C on 1/4 SDAY plates, to which small hyphal disks were attached for colony initiation. (B) Relative germination percentages of conidia after 24-h incubation at 25°C in a medium alone (control) or supplemented with Congo red (1 mg/mL). (C) Concentrations of protoplasts released from the hyphal cells after 6 h treatment with cell wall lysing enzymes in osmotic solution of 0.8 M sucrose. (D) Fluorescence intensity as an index of cell wall composition from flow cytometry of 2 × 10 4 conidia labeled with the fluorescent lectins ConA, GNL, and WGA. Asterisked bars in each bar group differ significantly from those unmarked (Tukey’s HSD, P < 0.05). Error bar: SD from three replicates.
Article Snippet: Carbohydrate epitopes on the surfaces of conidia were probed with the Alexa fluor 488-labeled
Techniques: Incubation, Fluorescence, Flow Cytometry, Labeling